Screening of Auramine O in Guci Tablets by HPLC
- VernacularTitle:HPLC法筛查骨刺片中的金胺O
- Author:
Zhang SHU
- Publication Type:Journal Article
- Keywords:
HPLC;
Guci tablets;
Auramine O;
Screening
- From:
China Pharmacist
2015;(1):172-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen auramine O in Guci tablets. Methods:Auramine O was detected by HPLC method on a Diamon-sil-C18(250mm×4.6mm,5μm)columnwiththemobilephaseofacetonitrile-0.025mol·L-1monopotassiumphosphatebuffersolu-tion(containing 0. 2% triethylamine,adjusting pH to 3. 0 with phosphoric acid)(36∶65)at the flow rate of 0. 8 ml·min-1. The detec-tion wavelength was 432 nm and the column temperature was 35℃. Results:The LOD of auramine O was 0. 05ng. Among 45 batches of tested samples, auramine O was found in seven ones. Conclusion:The method is simple, rapid and accurate, which can guarantee the drug safety of Guci tablets.
