Determination of geniposide in Diaojinyao powder by HPLC
10.3760/cma.j.issn.1673-4246.2015.01.018
- VernacularTitle:HPLC法测定吊筋药中栀子苷的含量
- Author:
Jun CHEN
;
Zhihao LI
;
Jing HE
- Publication Type:Journal Article
- Keywords:
Geniposide;
Diaojinyao powder;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2015;(1):70-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography (HPLC) method for the determination of geniposide in Diaojinyao powder. Methods The HPLC system consisted of the Fortis-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of HCN∶H2O (12∶88) , the flow rate was 1.0 ml/min, the column temperature was 30℃, The UV detector was set at 238 nm. Results The linear response range was 0.049~0.980mg/ml ( r=0.999 9). The average recovery of geniposide was 99.1%, and RSD was 1.66%. Conclusion The method is simple, rapid, accurate and repeatable. It can be applied in determination of Geniposide in Diaojinyao Powder.
