Determination of Content and Entrapment Efficiency of Ropivacaine Hydrochloride-loaded Multivesicular Liposomes
- VernacularTitle:盐酸罗哌卡因多囊脂质体含量及包封率测定
- Author:
Huilin ZENG
;
Shanshan WANG
;
Xudong FU
- Publication Type:Journal Article
- Keywords:
Ropivacaine hydrochloride;
Multivesicular liposomes;
Content;
Entrapment efficiency;
HPLC
- From:
China Pharmacist
2015;(1):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method for the content and entrapment efficiency of ropivacaine hydrochloride-loaded multivesicular liposomes. Methods: The separation of the multivesicular liposomes from the free drug was achieved by low-speed centrifugation. The concentration of ropivacaine hydrochloride in the supernatant and the multivesicular liposomes was determined by HPLC, and the entrapment efficiency was calculated. Results: The linear range of ropivacaine hydrochloride was 1. 0-80. 0μg· ml-1(r=0. 999 8). The average recovery was 99. 95% and RSD was 0. 72%(n=9). The content and entrapment efficiency of three batches of ropivacaine hydrochloride-loaded multivesicular liposomes was within the range of 99. 1%-100. 3% and 80. 06%-82. 14%, respectively. Conclusion:The method is simple and accurate, and can be used in the determination of content and entrapment efficien-cy of ropivacaine hydrochloride-loaded multivesicular liposomes.
