Therapeutic Effect of Extracts of Shengji Huayu Fang with Different Solvents in Rats with Deep Second De-gree Burn Wound
- VernacularTitle:生肌化瘀方不同溶剂提取物对大鼠深Ⅱ度烧伤创面的治疗作用
- Author:
Nian HUA
;
Ma MINGHUA
;
Zhu QUANGANG
;
Xu LINGLING
;
Li BING
- Publication Type:Journal Article
- Keywords:
Shengji Huayu Fang;
Wound healing;
Hydroxyproline;
DNA cell cycle
- From:
China Pharmacist
2015;(1):30-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the wound healing effects of the extracts of Shengji Huayu Fang with different solvents and demon-strate their important role in wound healing for deep second degree burn wound. Methods:Sixty Sprague-Dawley rats were randomly di-vidied into six groups(ten rats in each group): the control group (saline), the positive control group (asiaticoside group), Shengji Huayu Fang group respectively with petroleum ether, chloroform, ethyl acetate and n-butanol as the extraction solvent. The extracts of Shengji Huayu Fang with different solvents were applied in the rats with deep second degree burn wound, and the wound healing was e-valuated by the healing rate judged by naked eyes and computer image analysis, DNA cell cycle analysis using a flow cytometry, patho-logical reports and the degree of re-epithelialization studied by the other methods. Results:The mean healing time of the chloroform ex-tracts group [(15. 67 ± 1. 12)d] was much shorter than that of the control group [(22. 87 ± 1. 01)d, P<0. 01]. The hydroxyproline content and the percentage of S-phase cells in wound tissue in the chloroform extracts group was obviously higher than those in the con-trol group (P<0. 01). Conclusion:The present study indicates that topical application of chloroform extracts of Shengji Huayu Fang is beneficial to burn wound healing, and the chloroform extracts of Shengji Huayu Fang is the main bioactive fraction.
