Simultaneous Determination of Three Components in Compound Piracetam and Nimodipine Capsules by RP-HPLC-UV Wavelength Switching Method
- VernacularTitle:RP-HPLC-UV波长切换法同时测定复方吡拉西坦尼莫地平胶囊中3种成分的含量
- Author:
Jianying ZHAO
- Publication Type:Journal Article
- Keywords:
Compound piracetam and nimodipine capsules;
Captopril;
Aspirin;
Nimodipine;
HPLC;
Determination
- From:
China Pharmacist
2015;(1):41-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a RP-HPLC-UV wavelength switching method for the simultaneous determination of captopril, aspirin and nimodipine in compound piracetam and nimodipine capsules. Methods:The separation was carried out on a YMC-Pack Pro-C18 column(250 mm × 4. 6 mm,5μm) with acetonitrile-water(adjusting pH to 2. 5 with phosphpric acid)as the mobile phase with gra-dient elution. The flow rate was 1. 0 ml·min-1 . During 0-4. 3 min, the detection wavelength was 215 nm, during 4. 3-11. 0 min, the detection wavelength was 276 nm and during 11. 0-18. 0 min, the detection wavelength was 235 nm. The column temperature was 40℃. Results:The linear range of captopril, aspirin and nimodipine was 0. 054 7-1. 641 8 μg(r=0. 999 9),0. 055 3-1. 654 8 μg(r=0. 999 9) and 0. 077 7-2. 331 6 μg(r=0. 999 7), and the average recovery was 100. 69%(RSD=0. 69%,n=6),101. 04%(RSD=1. 05%,n=6)and 102. 56%(RSD=1. 14%,n=6), respectively. Conclusion: The method is simple, rapid and accurate, and can be used in the content determination of compound piracetam and nimodipine capsules.
