Effect of resveratrol as an antioxidant on oxidative damage to HepaRG cells induced by troglitazone
10.7644/j.issn.1674-9960.2014.12.008
- VernacularTitle:白藜芦醇对曲格列酮诱发HepaRG细胞氧化损伤的影响
- Author:
Zejun LI
;
Dan HU
;
Kezhao XIONG
;
Yue LIU
;
Xing FAN
;
Chunqi WU
;
Rigao DING
;
Xisha WANG
;
Quanjun WANG
- Publication Type:Journal Article
- Keywords:
resveratrol;
troglitazone;
reactive oxygen species;
antioxidant;
antioxidant enzymes
- From:
Military Medical Sciences
2014;(12):952-956
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of resveratrol ( Rev) as an antioxidant on oxidative damage to HepaRG cells induced by troglitazone ( Tro).Methods Cells were divided into five groups: control ( RPMI 1640 only with 0.1%DMSO), Tro(50 μmol/L), Tro(50 μmol/L) +Rev(15 μmol/L), Tro(50 μmol/L) +Rev(7.5 μmol/L) and Tro (50 μmol/L)+Rev(3.75 μmol/L) groups.MTT assay was performed to detect the viability of Rev-treated, Tro-treated and Rev with 50 μmol/L Tro-treated HepaRG cells.After 48 hours, the level of reactive oxygen species (ROS) and lipid oxidation ( malondialdehyde , MDA ) , degree of apoptosis , total antioxidant capacity , activity of hydrogenperoxidase (catalase, CAT), glutathione peroxidase (GSH-px) and superoxide dismutase(SOD)of these groups were identified. Results Tro could obviously cause HepaRG cells to produce oxidative stress .Compared with control group ,ROS and lipid peroxidation ( MDA) levels and the rate of apoptosis and necrosis in Tro-treated group were significantly increased ( P<0.05),total antioxidant capacity greatly reduced (P<0.05),and the activity of CAT,GSH-px and SOD was decreased (P<0.05).After adding various concentrations of Rev interaction , ROS and MDA production volume decreased (P < 0.05), and the apoptosis and necrosis rate correspondingly declined (P<0.05).Total antioxidant capacity of the cells and the activity of the three antioxidant enzymes were increased (P<0.05), and there was a dose-dependent relationship. Conclusion Tro can cause HepaRG cells to produce significant oxidative stress while Rev can significantly improve the oxidative damage of Tro to HepaRG cells .
