The Effects of Nonylphenol on Calcium Signaling and Cell Proliferation in H9c2 Cell and Its Mechanism
- VernacularTitle:壬基苯酚对H9c2心肌细胞钙信号和细胞增殖的影响及其作用机制
- Author:
Qinghua GAO
;
Shuice LIU
;
Feng GUO
;
Shuyuan LIU
;
Xiangchen YU
;
Wumaer XIAERBATI
;
Xuefei SUN
;
Nan MAO
;
Meimi ZHAO
;
Tong ZHU
;
Liying HAO
- Publication Type:Journal Article
- Keywords:
nonylphenol;
H9c2 cell;
calcium ion;
cell proliferation;
GPR30
- From:
Journal of China Medical University
2015;(2):109-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of nonylphenol(NP)on the intracellular calcium concentration changes and cell proliferation,and the involvement of GPR30 receptor in H9c2 cell. Methods The intracellular calcium concentration changes were recorded by using intracellular calcium determination method and cell proliferation was observed by MTT method in H9c2 cell. Results NP(1×10-10 mol/L)increased the intra?cellular calcium concentration changing amplitude and promoted the proliferation of H9c2 cells,while NP(1×10-6 mol/L)decreased intracellular calcium concentration changing amplitude and suppressed cell proliferation. G15 could block the promoting effect of 1×10-10 mol/L NP on the intracel?lular calcium concentration and cell proliferation,but could not block the inhibition of 1×10-6 mol/L NP on the intracellular calcium increase and cell proliferation. Conclusion The results indicate that NP affect rapid calcium signal changes and cell proliferation in non?monotonic dose dependent manner,and its mechanism may be due to the different involvement of GPR30 receptor in different concentrations.
