Inhibition of ursolic acid on angiogenesis and xenografts in zebrsfish (danio rerio)
10.3969/j.issn.1001-1978.2015.07.023
- VernacularTitle:熊果酸对斑马鱼血管生成及斑马鱼移植癌的抑制作用
- Author:
Xiqiang CHEN
;
Lifang CHENG
;
Xingang XU
;
Kechun LIU
;
Xuesheng YAN
;
Ximin WANG
;
Jin LIU
;
Ying LI
;
Hairong HOU
;
Dandan SUN
;
Liwen HAN
;
Weibing PENG
- Publication Type:Journal Article
- Keywords:
ursolic acid;
zebrafish;
anti-cancer;
an-giogenesis;
SMMC-7721 cell;
HT-29 cell
- From:
Chinese Pharmacological Bulletin
2015;(7):1004-1008
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the anti-angiogenesis and anti-xenograftes of UA in zebrafish larvaes. Meth-ods 24 hour post-fertilization ( hpf ) TG zebrafish was treated with different concentration of UA for 24h, and the number of intersegmental vessels( IVS) were detec-ted under fluorescent microscope respectively;then the models of AB/TG zebrafish xenografts were established by be micro-injected with SMMC-7721 or HT-29 cell at 48hpf respectively, and after cocultured with UA for 48h, optical density (OD) of the SMMC-7721 cell and subintestinal veins ( SIVs) induced by HT-29 were e-valuated under confocal microscope. Results ISV as-say showed that UA could cause IVS missing or disap-perance, inhibition ratio reaching 20. 25% and 26. 65%. UA blocked the spread of SMMC-7721 cells in zebrafish and OD value,and inhibition ratios at three concentrations were 38. 01%, 54. 69%, 61. 88%, re-spectively; in another SIVs assay induced by xeno-grafts, UA at concentration 10 and 15mg·L-1 showed that SIVs were inhibited (P<0. 01) obviously. Con-clusion UA could inhibit the angiogenesis and the growth of SMMC-7721/HT-29 xenografts,and the anti-tumor mechanism may be related with VEGFR2 expres-sion.
