Liensinine promotes apoptosis of bladder cancer 5637 cells and enhances Caspase-7 expression and activation
10.11958/j.issn.0253-9896.2015.07.006
- VernacularTitle:莲心碱促进膀胱癌5637细胞凋亡增强Caspase-7表达及活化
- Author:
Bin XU
;
Tianyu ZHANG
;
Ning TAN
;
Qi YAN
;
Lingli XU
;
Xiaojia LIU
;
Li GAO
- Publication Type:Journal Article
- Keywords:
urinary bladder neoplasms;
alkaloids;
cell proliferation;
apoptosis;
cysteine endopeptidases;
liensinine;
Caspase-7
- From:
Tianjin Medical Journal
2015;(7):724-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Liensinine on apoptosis of 5637 cells, and its mechanism thereof. Meth?ods CCK-8 method and the colony formation test were used to detect cell viabilities, and then inhibition rates were calcu?lated. Flow cytometry was used to detect the effects of Liensinine on apoptosis of 5637 cells. Western blot assay was used to detect Caspase-7 protein expression. Results CCK-8 assay and colony formation test indicated that Liensinine inhibited the cell proliferation significantly. Results of flow cytometry indicated that Liensinine induced early apoptosis of 5637 cells. Western blot assay showed that Liensinine improved the expression of Caspase-7 and enhanced the activation of Caspase-7 in 5637 cells. Conclusion Liensinine could inhibit the proliferation of 5637 cells, induce early apoptosis, which may be re?lated with the enhanced expression of Caspase-7 and its activation.
