Effects of MG132 on protein expression of SnoN and fibrosis-related in-dicators in NRK-52E cells after incubated with high concentration of glu-cose
10.3969/j.issn.1000-4718.2015.01.013
- VernacularTitle:M G132对高糖条件下肾小管上皮细胞 SnoN 蛋白表达及纤维化效应的影响
- Author:
Chunhua SHI
;
Mingjun SHI
;
Yuanyuan WANG
;
Lirong LIU
;
Changzhi ZHANG
;
Shuang LI
;
Ying XIAO
;
Rui YAN
;
Bing GUO
- Publication Type:Journal Article
- Keywords:
Renal tubular epithelial cells;
High glucose;
MG132;
SnoN protein;
Smad ubiquitination regula-tory factor 2;
Arkadia protein
- From:
Chinese Journal of Pathophysiology
2015;(1):64-68
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of proteasome inhibitor MG 132 on the expression of SnoN in renal tubule epithelial cells incubated in high glucose , and to explore the possible mechanism and function that MG 132 reduces or slows down renal tubular interstitial injury after incubated in high glucose .METHODS:The NRK-52E cells were divid-ed into normal control group (NG), high glucose group (HG) and high glucose plus pretreatment with different doses of MG132 group (HG+MG132).The immunofluorescence staining was used to detect the protein expression of E-cadherin and α-smooth muscle actin (α-SMA) in NRK-52E cells under different conditions .The relative protein expression levels of SnoN, Smad ubiquitination regulatory factor 2 (Smurf2), Arkadia, E-cadherin, α-SMA and collagen type Ⅰ(Col-Ⅰ) were detected by Western blotting .RESULTS:Compared with NG group , the expression of E-cadherin and SnoN was de-creased (P<0.05), while the expression of α-SMA, Col-Ⅰ, Smurf2 and Arkadia was increased (P<0.05).Compared with HG group, the protein expression of SnoN and E-cadherin was significantly up-regulated in HG+MG132 group ( P<0.05 ) , and the protein expression of α-SMA and Col-Ⅰwas significantly down-regulated in a dose-depended manner ( P<0.05).However, no effect on the protein expression of Smurf2 and Arkadia was observed.CONCLUSION: MG132 in-hibits the degradation of SnoN protein induced by high glucose , thus reducing the renal fibrosis .
