Screening and primary analysis of microRNAs expression profile in human breast cancer BT474 cells with different lever of HER-2

VernacularTitle:筛选并初步分析不同 HER-2水平的人乳腺癌 BT474细胞内miRNAs差异性表达
Author: Guilu ZHU ; Chengchen XU ; Zhengsheng WU ; Qiang WU
Publication Type:Journal Article
Keywords: breast neoplasm; miRNAs; HER-2
From: Chinese Journal of Clinical and Experimental Pathology 2014;(11):1206-1210
CountryChina
Language:Chinese
Abstract: Purpose To detect the expression profile of microRNAs ( miRNAs) in human breast cancer cells BT474 and BT474/ siR-NA HER-2, and to screen out miRNA expression profile related to HER-2. Methods RNA interference was used to inhibit HER-2 expression in human breast cancer line BT474. The expression profile of miRNAs was detected by miRNA microarray technique. Re-sults According to discrepancy power >2. 0 and P value<0. 05, there were 338 miRNAs related to HER-2, including 110 up-regu-lated and 228 down-regulated miRNAs. Conclusion miRNA microarrays can detect the differentially expressed miRNAs in human breast cancer BT474 cells with different level of HER-2. The miRNAs related to HER-2 which play a role in breast cancer should be found after further analysis.