MicroRNA-21 for regulation of TLR4 in Hela cells
10.3969/j.issn.2095-4344.2015.02.011
- VernacularTitle:在Hela细胞中验证miRNA-21对TLR4的调控
- Author:
Jing ZHAO
;
Peng YUE
;
Jiafang HUANG
;
Yutao WANG
;
Ting MA
;
Baorong CHEN
- Publication Type:Journal Article
- Keywords:
Genes;
Tol-Like Receptor 4;
Hela Cells;
MicroRNAs
- From:
Chinese Journal of Tissue Engineering Research
2015;(2):220-224
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:MicroRNAs (miRNA) through regulating specific target gene mRNA expression play important roles in different processes of diseases. OBJECTIVE:To study the interaction of miRNA-21 with its target gene TLR4 in Hela cels. METHODS:The candidate target gene of miRNA-21 was determined according to miRNA analysis databases. The constructed recombinant adenovirus vector carrying pri-miRNA-21 gene was used, which could package and amplify viruses to transfect Hela cels. Then, the expression of fluorescent proteins was detected. Forty-eight hours after transfection of miRNA-21 or control, extracted proteins were used for detection of TLR4 protein using western blot assay. RESULTS AND CONCLUSION:Recombinant adenoviruses pAd/pri-miRNA-21 and pAd/neg at 100 MOI could successfuly infect Hela cels. Bioinformatic analysis suggested several possible binding sites between miRNA-21 and TLR4. The experimental results showed that miRNA-21 down-regulated TLR4 at protein levels, indicating that miRNA-21 can interfere with the expression of TLR4 target gene.
