Expression of adiponectin in the periodontal tissue of Kunming mice with diabetic periodontitis
10.3969/j.issn.2095-4344.2015.05.012
- VernacularTitle:糖尿病牙周炎昆明系模型小鼠牙周组织中脂联素的表达
- Author:
Bo ZHAO
;
Yonglan WANG
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Adiponectin;
Periodontitis;
Diabetes
- From:
Chinese Journal of Tissue Engineering Research
2015;(5):721-726
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Adiponectin and periodontal disease are closely related with type 2 diabetes. OBJECTIVE:To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis. METHODS:Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods:control group (normal feeding), diabetes group (diabetic model was established by injection of al oxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats. RESULTS AND CONCLUSION:At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P<0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P<0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present in the control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.
