MicroRNA expression profiling in male and female model mice after renal ischemia-reperfusion injury
10.3969/j.issn.2095-4344.2015.05.020
- VernacularTitle:不同性别肾缺血再灌注损伤模型小鼠MicroRNA的表达谱
- Author:
Weiwei TANG
;
Xiaoqing XI
;
Honglin HU
;
Yawei HUANG
;
Zhenfeng YE
;
Dingyi CHEN
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Kidney;
Reperfusion Injury;
Microchip Analytical Procedures
- From:
Chinese Journal of Tissue Engineering Research
2015;(5):772-777
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Renal ischemia-reperfusion injury has been shown to exhibit gender difference, but its precise mechanisms deserve further investigations. OBJECTIVE:To investigate the differential expression of microRNAs in the kidney between female and male mice in order to study the effects and mechanisms of microRNA in pathogenesis of ischemia-reperfusion injury between different genders. METHODS:Male and female mice received kidney ischemia for 45 minutes and reperfusion injury for 24 hours. Simultaneously, male and female sham surgery groups served as controls. The microRNA gene chip technology was used to detect the differences of microRNA expression in the kidney of male and female mice at 45 minutes after ischemia and 24 hours of reperfusion as wel as after sham surgery. The threshold of difference in expression among samples was double. RESULTS AND CONCLUSION:Five microRNAs were up-regulated between female and male ischemia-reperfusion injury groups. Twenty-nine microRNAs differential y expressed in the female ischemia-reperfusion group and female sham surgery group, including 25 up-regulated microRNAs and 4 down-regulated microRNAs. Thirty-eight microRNAs differential y expressed in male ischemia-reperfusion injury group and male sham surgery group, including 9 up-regulated microRNAs and 29 down-regulated microRNAs. 102 microRNAs differential y expressed in the female sham surgery group and male sham surgery group, including 22 up-regulated microRNAs and 80 down-regulated microRNAs. Results suggested that there was differential expression in microRNAs in the kidney before and after renal ischemia-reperfusion in male and female mice. These differential y expressed microRNAs may be lead to different sensitivity and tolerance to the ischemia-reperfusion injury in the kidney of male and female mice.
