Cross-reactivity of tree shrew sera with various secondary antibodies:extensive application to tree shrew models of diseases
10.3969/j.issn.2095-4344.2015.05.024
- VernacularTitle:树鼩血清与多种二抗的交叉反应:可在树鼩疾病动物模型中广泛应用
- Author:
Guangping RUAN
;
Xiang YAO
;
Jufen LIU
;
Jinxiang WANG
;
Jie HE
;
Jianyong YANG
;
Xinghua PAN
- Publication Type:Journal Article
- Keywords:
Tupai dae;
Receptors,IgG;
Cross Reactions
- From:
Chinese Journal of Tissue Engineering Research
2015;(5):794-798
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Tree shrew is a representative between insectivore and primates, has a high degree of evolution, is more inexpensive primates, has high use of medical biology, and has been attached by scholars. OBJECTIVE:To detect whether the commonly used secondary antibodies have immune response with tree shrew serum. METHODS:Western blot assay and enzyme linked immunosorbent assay were utilized to detect whether the tree shrew serum had cross-reacts with anti-rabbit, anti-goat, anti-human, anti-mouse, anti-rat, and anti-monkey secondary antibodies. RESULTS AND CONCLUSION:Western blot assay results indicated that tree shrew serums did not react with anti-rabbit, anti-goat, anti-human, anti-mouse, and anti-rat secondary antibodies and had cross reaction with anti-monkey secondary antibody. Enzyme linked immunosorbent assay results also indicated that tree shrew serums were cross-reactive with anti-monkey secondary antibody, but did not have cross-reactivity with the other secondary antibodies. Above data confirmed that the usual y soled secondary antibody cannot be used to immunoassay with tree shrews IgG. Only anti-monkey secondary antibody has cross-react with tree shrew serum. It is necessary to prepare anti-tree shrew IgG monoclonal and polyclonal antibodies. When no antibody is readily available at present, anti-monkey secondary antibody can be used to substitute detection, and can be widely applied in the study of tree shrew models of disease.
