Experimental research on the characteristics of pancreatic cells regeneration in an animal model for isolated pancreatic trauma
10.3969/j.issn.1671-8348.2015.04.004
- VernacularTitle:单纯性胰腺创伤后大鼠胰腺细胞增殖状况的实验研究
- Author:
Guangyu CHEN
;
Ruiwu DAI
;
Hao LUO
;
Zhengyu CHEN
;
Tao CHEN
;
Dongxuan LI
;
Runhua LV
;
Lijun TANG
- Publication Type:Journal Article
- Keywords:
model,animal;
cell regeneration;
pancreatic trauma;
Bcl-2;
Bax
- From:
Chongqing Medicine
2015;(4):442-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationships between tissue damage and the ability of the pancreatic cells to regenerate ,and analyze the alteration of the pancreatic cells regeneration .Methods Sixty rats were divided into two groups :impact group(the pan‐creas was injured by a BIM‐Ⅲ biotical impact machine ,40 rats) and control group(sham operated ,20 rats) .All rats were sacrificed at 6 h ,24 h ,72 h ,7 d after operation .The level of AMS ,LPS in the serum were detected by spectrophotometry ,pancreatic cells re‐generation were examined and analyzed by TUNEL staining and flow cytomertry ,and the Bcl‐2 and Bax expression were measured by Western blot .Results In the impact groups ,LPS was activated later than AMS ,and lasted persistently .The results from TUNEL stain ,flow cytometry and Western blot indicated that pancreatic trauma induces cell death and the compensatory prolifera‐tion of pancreatic cells .The characteristics of pancreatic cells regeneration in the animal model of isolated pancreatic trauma indicate that the proper remedial time is in the first 24h after the pancreatic trauma .Conclusion Detecting AMS and LPS at the same time can help us to determine the exocrine function of pancrease .
