Analysis of the contents of isorhynchophylline in different medicinal parts of Ramulus Uncariae Cum Uncis
10.3760/cma.j.issn.1673-4246.2015.03.018
- VernacularTitle:中药钩藤不同药用部位异钩藤碱含量分析
- Author:
Meng WANG
;
Yaxin ZHAO
- Publication Type:Journal Article
- Keywords:
Ramulus Uncariae Cum Uncis;
Medicinal parts;
Content analysis;
Isorhynchophylline
- From:
International Journal of Traditional Chinese Medicine
2015;(3):258-260
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the content of isorhynchophylline in different medicinal parts of Ramulus Uncariae Cum Uncis.MethodsHPLC was adopted to determine 8 batches of different Ramulus Uncariae Cum Uncis of different origin and different medicinal parts. The Waters Symmetry C18 color(4.6 mm×250 mm, 5μm) was used with mobile phase of methanol -0.01 mol/L ammonium acetate buffer(pH 8.0)(60∶40), column temperature of 25℃ , 20μl sample volume, velocity of 1.0 ml/min, and detection wavelength of 246 nm. ResultsIsorhynchophylline can be detected in all 8 batchs of Ramulus Uncariae Cum Uncis in different regions, and different content were found among different origins. The content of isorhynchophylline in different parts of the same origin showed a decreasing sequence of the rhabd, stem with hook, stem without hook, and twig without hook and leaves.ConclusionMost of medicinal part of Ramulus Uncariae Cum Uncis contains isorhynchophylline, which provide a lab basis for exploring medicinal parts of this herbal medicine.
