Expression of Cdh1 and its downstream substrates in primary neurons after oxygen-glucose deprivation
10.3969/j.issn.2095-4344.2015.05.005
- VernacularTitle:原代缺氧缺糖损伤神经元模型Cdh1及其下游底物的表达
- Author:
Wei QIAN
;
Jin QIU
;
Yuehong QI
;
Wenlong YAO
;
Xue ZHANG
;
Chuanhan ZHANG
- Publication Type:Journal Article
- Keywords:
Stem Cel s;
Anoxia;
Neurons;
Cyclins
- From:
Chinese Journal of Tissue Engineering Research
2015;(5):681-684
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Cdh1 has been shown to express in rat hippocampus and cortex in a large number. Moreover, in vitro test demonstrated that Cdh1 expression was higher in neurons than in neural stem cel s, which possibly associated with the differentiation of neural stem cel s into neurons. However, the effects of anaphase promoting complex Cdh1 on ischemic neuronal damage remain unclear.
OBJECTIVE:To investigate the expression of Cdh1 and its downstream substrate in primary cultured neurons with oxygen-glucose deprivation. METHODS:Primary neurons from cortex of postnatal 24-hour rat pups were cultured in vitro, and identified by immunofluorescence staining. The oxygen-and glucose-deprived models were established by three gas incubator fil ed with nitrogen in sugar-free Earle’s solution. After 1 hour of hypoxia, reoxygenation was conducted. Real-time fluorescent quantitative PCR was used to detect the mRNA expression of Cdh1 and its downstream substrates Skp2, Cyclin B1 before hypoxia, 6 hours, 1, 3, 7 days after oxygen glucose deprivation. RESULTS AND CONCLUSION:After oxygen glucose deprivation, the expression of Cdh1 and Cyclin B1 in primary neurons was increased (P<0.05), while Skp2 expression was decreased (P<0.05). Above data indicated that Cdh1 expression in neurons increased after oxygen-glucose deprivation. It may degrade Skp2 and participate in hypoxic neuronal apoptosis by ubiquitination.
