Expression of DNA methyltransferase mRNA in children with immune thrombocytopenia

VernacularTitle:儿童免疫性血小板减少症DNA甲基转移酶mRNA的表达
Author: Aiju XIAO ; Tuanjie WANG ; Lijia CAO ; Taixin SHI ; Dongju ZHAO ; Peiling LI ; Ruijuan REN
Publication Type:Journal Article
Keywords: immune thrombocytopenic purpura; DNA methyltransferase 1; DNA methyltransferase 3a; child
From: Journal of Clinical Pediatrics 2015;(2):147-150
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between DNA methylation and pathogenesis of childhood immune thrombocytopenic purpura (ITP) by examining the expression of DNA methyltransferase 1(Dnmt1) and DNA methyltransferase 3a (Dnmt3a) mRNA in peripheral blood lymphocytes of the children with ITP. Methods Expression of Dnmt 1 and Dnmt3a mRNA in the peripheral blood lymphocytes in 36 children with newly diagnosed ITP and 26 healthy children were detected using RT-PCR. Results Dnmt1 mRNA expression in peripheral blood lymphocytes in children diagnosed with ITP was 3.02±0.49, significantly lower than 4.58±0.52 in the control group (t=11.95, P<0.001). Dnmt3a mRNA expression in peripheral blood lymphocytes in children diagnosed with ITP was 1.49±0.44, signiifcantly lower than 2.41±0.32 in the control group (t=9.12, P<0.001). Conclusions Children with newly diagnosed ITP have lower DNA methylation status in peripheral blood lymphocytes as compared to that in healthy children. The DNA methylation may play an important role in the etiology of acute ITP in children.