Cloning of the Coding Region and 3′Non-coding Region of Calmodulin 2 in Guinea Pig
- VernacularTitle:豚鼠钙调蛋白2基因编码区及3′端非编码区序列克隆
- Author:
Rui FENG
;
Yan LIU
;
Lei YANG
;
Huiyuan HU
;
Feng GUO
;
Meimi ZHAO
;
Jinsheng ZHAO
;
Liying HAO
- Publication Type:Journal Article
- Keywords:
calmodulin;
gene cloning;
sequence;
coding region
- From:
Journal of China Medical University
2015;(2):123-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone the coding region and 3′non?coding region of calmodulin 2(CaM2)in guinea pig,to provide the genetic informa?tion for studying the gene function of Calmodulin 2. Methods Total RNA was extracted from heart tissue of guinea pig,the coding region and 3′non?coding region of CaM2 were amplified by RT?PCR and 3′?RACE PCR methods,and the recombinant plasmid was constructed by inserting cDNA of the coding region and 3′non?coding region of CaM2 into the cloning vector by genetic engineering technology followed by DNA sequencing and se?quence analysis. Results The cloned coding region of CaM2 was 450 bp,and the 3′non?coding region of CaM2 was 660 bp. The amino acid se?quences of the coding region of CaM2 was consistent with those of other CaM subtypes,and the 3′non?coding region of CaM2 had low homology with those of other subtypes. Conclusion The cloning of CaM2 coding region and 3′non?coding region in guinea pig was the foundation for further study on the gene function of CaM2 and its role in related diseases.
