The impact ofβ-lactam antibiotics on infants′intestinal flora detected by using 16S rRNA quantitative PCR technique
10.3969/j.issn.1673-4130.2015.03.030
- VernacularTitle:应用16SrRNA荧光定量PCR技术研究β-内酰胺类抗菌药物对婴儿肠道菌群的影响
- Author:
Rui HE
;
Chong ZHANG
;
Guoping ZHANG
;
Cuisheng ZHAO
- Publication Type:Journal Article
- Keywords:
intestinal flora;
antibiotics;
16S rRNA;
infant
- From:
International Journal of Laboratory Medicine
2015;(3):357-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of β-lactam antimicrobial drugs on infants′intestinal flora.Methods Infants from 0 to 1 years old were enrolled in the study,who′s feces samples were tested for Bacillus bifidus,lactobacillus,enterococcus and E.coli by using 16S rRNA quantitative PCR technique before using antibiotics,on the third and fifth day duringβ-lactam antibiotics treatment and the seventh day after treatment.Results The numbers of Bacillus bifidus,lactobacillus,E.coli and enterococci detec-ted in usingβ-lactam antibiotics group were not statistically different from those in not using antibiotics group(P >0.05).The num-bers of Bacillus bifidus and lactobacillus increased with the treatment process,the differencies was statistically significant(P <0.05).The recovery of the numbers of intestinal Bacillus bifidus and lactobacillus in not using antibiotics group was significantly faster than using antibiotics group,the difference was statistically significant(P <0.05).Conclusion β-lactam antibiotics have gen-eral killing effect on the intestinal flora in infants,which could recover to normal.The recovery of intestinal flora could be delayed if antibiotics are used,however,reasonable antibiotics treatment would be very helpful in the treatment of primary diseases.
