The effect of fluoride on the viability of rat ameloblast HAT-7 cells and Ca2 + concentration in the cells
10.3969/j.issn.1001-3733.2015.01.01
- VernacularTitle:氟对体外培养大鼠成釉细胞 HAT-7细胞活性和对细胞内 Ca2+浓度的影响
- Author:
Lin MA
;
Ying ZHANG
;
Ming ZHONG
;
Li ZHU
;
Kaiqiang ZHANG
;
Hefeng GU
;
Lu LIU
;
Siyu ZHANG
;
Ruibo CHENG
- Publication Type:Journal Article
- Keywords:
Fluoride;
Ameloblast;
CCK8;
Flow cytometry;
Ca2 +;
Laser scanning confocal microscopy
- From:
Journal of Practical Stomatology
2015;(1):7-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of fluoride on the viability of rat ameloblast HAT-7 cells and calcium concentration in the cells.Methods:HAT-7 cells were exposed to NaF at 0,0.4,0.8,1.6,3.2 and 6.4 mmol/L for 24,48 and 72 h respectively. CCK-8 assay was performed to examine the cells proliferation;the apoptosis rate was determined by flow cytometry;Ca2 +concentration in the cells was detected by laser scanning confocal microscopy.Results:The cell proliferation was increased by NaF at 0.4 mmol/L and 0.8 mmol/L,whereas inhibited at 1.6 mmol/L and above.The effects were in a time-dependent manner.NaF increased apoptosis of the cells and increased Ca2 + concentration in the cells in a concentration-dependent manner.Conclusion:Fluoride at low doses promotes proliferation,at high doses inhibits proliferation of HAT-7 cells.NaF of 1.6 mmol/L or more induces apoptosis of HAT-7 cells and in-duce Ca2 + overloading in the cells.
