Expressions of antisense non-coding RNA in INK4 locus and tumor suppressors in peripheral blood lymphocytes of patient with cirrhosis and hepatocellular carcinoma
10.3969/j.issn.1008-9691.2015.01.021
- VernacularTitle:肝硬化和肝癌患者外周血淋巴细胞中INK4位点反义非编码RNA和肿瘤抑制因子的表达
- Author:
Hao LIN
;
Chusheng ZHAO
;
Yongping ZHENG
- Publication Type:Journal Article
- Keywords:
Cirrhosis;
Hepatocellular carcinoma;
Lymphocyte;
Antisense non-coding RNA in the INK4 locus;
p14ARF;
p15INK4b;
p16INK4a
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2015;(1):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical diagnostic and differential diagnostic values of antisense non-coding RNA in the INK4 locus (ANRIL) and tumor suppressors (p14ARF, p15INK4b and p16INK4a) mRNA expression levels in peripheral blood lymphocytes of patients with cirrhosis and hepatocellular carcinoma. Methods The patients with hepatocellular carcinoma and cirrhosis admitted in Shantou Central Hospital from October 2013 to April 2014 were selected. The real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect ANRIL, p14ARF, p15INK4b and p16INK4a mRNA expression levels of peripheral blood lymphocytes. The subjects having taken physical health examinations in outpatient clinics were assigned in the healthy control group. Results During the study period, 19 cases of hepatocellular carcinoma, 24 cases of cirrhosis, and 31 healthy controls were finally enrolled. In the hepatocellular carcinoma group, the mRNA expression level of ANRIL was significantly higher than that of the healthy control group (?Ct:13.07±0.62 vs. 12.45±0.84, P<0.01), while p15INK4b mRNA expression level was obviously lower than that of the healthy control group (13.24±0.98 vs. 13.99±0.99, P<0.05). But there were no significant differences in the mRNA expression levels of ANRIL (13.07±0.65 vs. 12.71±0.76) and p15INK4b (13.24±0.98 vs. 13.55±1.08) between the groups of hepatocellular carcinoma and cirrhosis (both P>0.05). There were also no statistically significant differences in p14ARF and p16INK4a mRNA expressions among the three groups (all P>0.05). Conclusion The elevation of ANRIL and descent of p15INK4b mRNA expression levels in peripheral blood lymphocytes in patients with liver lesion can be used as the reference indicators for the early diagnosis of hepatocellular carcinoma and to predict their prognoses.
