Comparison of osteopontin and mineralization culture medium in the induction of dental pulp stem cells into osteoblasts

VernacularTitle:骨桥蛋白与矿化液诱导牙髓干细胞向成骨细胞分化的对比研究
Author: Xin SHI ; Pengfei ZHANG ; Mengtong YUAN ; Mingyue LIU ; Weiping HU
Publication Type:Journal Article
Keywords: Osteopontin; Dental pulp stem cells; Osteoblasts; Differentiation
From: Journal of Practical Stomatology 2015;(1):11-14
CountryChina
Language:Chinese
Abstract: Objective:To compare the osteogenic differentiation of dental pulp stem cells(DPSCs)induced by osteopontin(OPN)and mineralizing culture medium(MCM).Methods:DPSCs were cultured with OPN(OPN group)and MCM(MCM group)respectively. The morphology of the DPSCs were observed under inverted microscope.The mineralize nodules were observed by alizarin red staining. RT-RCR was used to detect the mRNA expression of bone sialoprotein (BSP),Runt-related transcription factor 2(Runx-2),osteocal-cin(OCN)and collagen-1(Col-1).Results:Similar number of mineralized nodules was found in the 2 groups(P >0.05)after 28 day culture.The mRNA expression level of BSP gene in OPN group was higher than that in MCMgroup(0.864 ±0.112 and 0.514 ±0.068, P <0.05),while the expression level of Runx-2 gene in OPN group is lower than that in MCMgroup(0.186 ±0.017 and 0.324 ±0. 058,P <0.05).The expression level of Col-1 and OCN genes in both groups were similar(P >0.05).Conclusion:The capabilities of OPN and MCMin inducing osteogenic differentiation of DPSCs are similar.