Effect of Different Acupuncture Stimuli on Uterine Micro-circulation and Circulation-related Substances of Dysmenorrheal Rats with Cold Stagnation Syndrome
10.3969/j.issn.1005-5304.2015.03.014
- VernacularTitle:不同针刺刺激量对寒凝证类痛经大鼠子宫微循环及其相关物质的影响
- Author:
Qingqing ZHANG
;
Shipeng ZHU
;
Li LUO
;
Mengwei GUO
;
Ling ZHANG
;
Songxi SHEN
;
Dandan QI
;
Jiamin YANG
;
Xiaoyu SHEN
;
Yongsi XU
;
Yang WANG
;
Yafang ZHAO
;
Bo JI
;
Xiaoxuan REN
;
Lufen ZHANG
;
Jiang ZHU
;
Xiaohong LI
- Publication Type:Journal Article
- Keywords:
different acupuncture stimuli;
dysmenorrheal rats with cold stagnation syndrome;
uterine micro-circulation;
TXB2;
6-keto-PGF1α
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):51-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of different acupuncture stimuli on uterine micro-circulation of dysmenorrheal rats with cold stagnation syndrome. Methods Totally 32 three-month old female SD rats in diestrus were randomly divided into saline control group, model group, A stimuli group, and B stimuli group, 8 rats in each group. Model group and treatment groups were given whole body freezing combined with estradiol benzoate injection method to establish models. A stimuli group was given deep puncture with manipulation, while B stimuli group was treated by shallow puncture without manipulation. Diameter of uterine capillary,micro-vessel, TXB2, and 6-keto-PGF1αlevels were observed in each group. Results Compared with the saline group, capillary diameter in model group was significantly reduced at 5, 10, 20, 30 min time point (P<0.01);micro-vascular diameter was significantly reduced at 5, 10, 20, 30, 40 min time point (P<0.01);plasma 6-keto-PGF1α levels decreased (P<0.01);TXB2/6-keto-PGF1αincreased significantly (P<0.01). Compared with model group, capillary diameter in A stimuli group enlarged at 5, 10, 20, 30 min time point (P<0.05), micro-vascular diameter dilated at 5, 10, 20, 30, 40 min time point (P <0.01), plasma 6-keto-PGF1α level increased (P <0.05), TXB2/6-keto-PGF1αdecreased significantly (P<0.05);micro-vascular diameter in B stimuli group dilated at 20, 30 min time point (P<0.05), plasma TXB2/6-keto-PGF1α decreased significantly (P<0.05). Compared with B stimuli group, capillary diameter in A stimuli group dilated at 5, 10, 20, 30 min time point (P<0.05) and micro-vascular diameter dilated at 5, 10, 20, 30, 40 min time point significantly (P<0.01). Conclusion Dysmenorrheal rats with cold stagnation syndrome show obvious disorder of the uterus micro-circulation and circulation related substances. Both A and B acupuncture stimuli improved uterus micro-circulation of dysmenorrheal rats with cold stagnation syndrome, and its mechanism may be related to the recovery the balance between TXB2 and 6-keto-PGF1α.
