Effects of Soothing Liver and Activating Blood Chinese Medicine on Myocardial Cell Apoptosis and Related Gene Expression of BMSCs Transplanting Myocardial Ischemia Reperfusion Injury Rats
10.3969/j.issn.1005-5304.2015.03.015
- VernacularTitle:疏肝活血中药对骨髓间充质干细胞移植心肌缺血再灌注损伤大鼠心肌细胞凋亡及相关基因表达的影响
- Author:
Xinhui LI
;
Zhengde HUANG
;
Jianfang DU
;
Xuejiao XIE
;
Liqing SU
;
Yirong HE
- Publication Type:Journal Article
- Keywords:
soothing liver and activating blood Chinese medicine;
BMSCs;
IRI;
myocardial cell apoptosis;
protein expression;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of soothing liver and activating blood Chinese medicine on myocardial cell apoptosis and related gene expression of BMSCs transplanting on myocardial ischemia reperfusion injury (IRI) of rats;To discuss its mechanism of protecting myocardium. Methods Model of myocardial IRI was established in rats. BMSCs were isolated, cultivated, and transplanted in IRI rats. SD rats were randomly divided into sham-operation group, IRI group, BMSCs group, and combined group. Rats in combined group received gavage with soothing liver and activating blood Chinese medicine, while rats in other groups received gavage with the same dose of normal saline. After 4 weeks, myocardial cell apoptosis, Bcl-2, and Bax protein expression in myocardial cells were detected by TUNEL method and immunohistochemical method. Results Compared with IRI group, myocardial cell apoptosis index in the combined group and BMSCs group was lower, Bax expression decreased, Bcl-2 expression significantly increased (P<0.01);Compared with BMSCs group, myocardial cell apoptosis index in the combined group was lower;Bax expression decreased, Bcl-2 expression increased (P<0.05, P<0.01). Conclusion Soothing liver and activating blood Chinese medicine can inhibit BMSCs transplantation in IRI rat myocardial cell apoptosis, promote myocardial regeneration, and protect myocardial cells.
