Effect of Xiaotan Sanjie Recipe on Proliferation and Apoptosis of HCT116 Cell and Expression of Caspase3 Protein
10.3969/j.issn.1005-5304.2015.03.016
- VernacularTitle:消痰散结方对人结直肠癌HCT116细胞增殖和凋亡及Caspase3蛋白表达的影响
- Author:
Yuqi ZHOU
;
Min YE
;
Can LV
;
Pinkang WEI
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
Xiaotan Sanjie Recipe;
cell proliferation;
cell apoptosis;
Caspase3
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):60-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Xiaotan Sanjie Recipe (XTSJR) on the proliferation and apoptosis of colorectal cancer cell;To study its relevant mechanism. Methods The original XTSJR aqueous solution was lyophilized, weighted, and dissolved in cell culture and stored. HCT116 cell line was treated with different concentrations of XTSJR for 72 h, and the cell proliferation was detected by CCK-8 assay. XTSJR treated HCT116 cell line with the concentration of 0.94 mg/mL and 1.88 mg/mL for 24 h, 48 h, 72 h, and the cell proliferation was detected by CCK-8 assay. HCT116 cell line was treated with different concentrations (0.24, 0.47, 0.94, 1.88 mg/mL) of XTSJR for 72 h. The cell apoptosis was detected by TUNEL assay under the fluorescence microscope, and the post-intervention expression of Caspase3 protein was detected by Western blot. Results The inhibitory effects of XTSJR on the proliferation of HCT116 cell line was concentration-time dependent. The IC50 value at 72 h-time point was 0.94 mg/mL. Medicine concentration and the treated time have interactive contribution to the inhibitory effect. XTSJR induced the cell apoptosis. The apoptosis rate increased with the increasing medicine concentration. There was statistically significant difference compared with the control group ( P<0.01). After treatment the expression of Pro-Caspase3 decreased, while the expression Cleaved Caspase3 protein increased. Conclusion XTSJR inhibits the proliferation of HCT116 cell line and induce apoptosis. Its mechanism might be related to the activity of Caspase3 protein.
