Effects of Zuogui Jiangtang Yishen Decoction on Expressions of Glucose Regulated Protein78 and C/EBP Homology Protein in Podocyte of MKR Mice with Diabetic Nephropathy
10.3969/j.issn.1005-5304.2015.03.017
- VernacularTitle:左归降糖益肾方对MKR糖尿病肾病小鼠足细胞葡萄糖调节蛋白78、C/EBP同源蛋白表达的影响
- Author:
Yuan TANG
;
Rong YU
;
Wenjuan LUO
;
Cong CHEN
;
Yongjun WU
;
Jing ZENG
;
Xiang ZHANG
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
Zuogui Jiangtang Yishen Decoction;
MKR mice;
endoplasmic reticulum stress;
podocyte;
GRP78;
CHOP
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):65-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the specific expression of endoplasmic reticulum stress-related GRP78 and CHOP in podocyte of MKR mice with diabetic nephropathy (DN);To discuss the intervention effect of Zuogui Jiangtang Yishen Decoction (ZGJTYS). Methods 8-week old MKR mice were randomly divided into five groups:blank group, model group, ZGJTYS group, 4-Phenylbutyric acid group, gliquidone+benazepril group, and normal control group consisting of wild type C57BC/6 mice, 10 mice per group. All mice from model group and each treatment group received high-fat diet feed and unilateral nephrectomy to make the DN model. Mice from treatment groups received medicine intervention for four weeks. The levels of FBG were detected by electrochemical detection method, and the UmAlb was detected by ELISA. The expressions of GRP78, CHOP mRNA, and protein were detected by RT-PCR and Western blot. The morphological structure changes of the podocytes were observed by transmission electron microscopes. Results Compared with blank group, FBG and UmAlb in the model group increased significantly (P<0.01);the expressions of GRP78 mRNA and protein markedly decreased (P<0.01);the expressions of CHOP mRNA and protein increased (P<0.01). Compared with the model group, FBG and UmAlb in each treatment group significantly decreased (P<0.01);the expressions of GRP78 mRNA and protein in the ZGJTYS group and 4-Phenylbutyric acid group increased (P<0.01), the expressions of CHOP mRNA and protein decreased (P<0.01). The renal pathological lesions of each treatment group were significantly improved compared with model group. Conclusion ZGJTYS Decoction can effectively improve the damaged podocyte induced by endoplasmic reticulum stress, delay DN progress.
