Effects of Wuling Powder on Insulin Resistance of Mice Induced by High Lipid Diet
10.3969/j.issn.1005-5304.2015.03.019
- VernacularTitle:五苓散对高脂膳食诱导小鼠胰岛素抵抗的影响
- Author:
Yang YANG
;
Dan WANG
;
Chufeng YANG
;
Junyan WANG
;
Qiuhua ZHANG
- Publication Type:Journal Article
- Keywords:
Wuling Powder;
high lipid diet;
insulin resistance;
fasting plasma glucose;
insulin sensitivity index;
insulin resistance index;
mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):73-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Wuling Powder on insulin resistance of C57BL/6J mice induced by high lipid diet, and discuss the mechanism. Methods C57BL/6J mice were randomly divided into six groups:normal group, model group, rosiglitazone group, Wuling Powder in low, middle, and high dose groups, 10 mice per group. Besides the normal group, other five groups were fed with high fat and sugar diet, with a purpose to establish insulin resistance model. Normal group and model group were given pure water. Rosiglitazone group received a gavage with rosiglitazone of 0.75 mg/kg. Wuling Power low, middle, and high groups received gavage with Wuling Power of 1.23, 3.69, 11.07 g/kg, respectively, the does volume was 0.2 mL/10 g, once a day. The weight and abdominal girth were detected every week. At the end of the sixth week, mice were given 12-hour fasting, and their eyeball were taken for blood. The body weight, length, and fat in abdomen and both kidneys were detected. Paraffin section was made with HE staining. FPG and FINS of each group were detected. ISI and IRI were calculated, and TC and TG were detected. Results Compared with the model group, Wuling Powder can significantly reduce the body weight and abdominal girth of mice (P<0.01, P<0.001), improve liver fatty degeneration, lower the FPG, FINS, TCH, TG, IRI, and increase the ISI in mice (P<0.05, P<0.01). Conclusions Wuling Powder has the effect of preventing insulin resistance of C57BL/6J mice induced by high lipid diet.
