Study on Anti-oxidant Activity Parts of Ferula sinkiangensis K. M. Shen
10.3969/j.issn.1005-5304.2015.03.021
- VernacularTitle:新疆阿魏抗氧化活性部位研究
- Author:
Haiying ZHANG
;
Longlong ZHOU
;
Lin JIANG
;
Mingxin ZHOU
- Publication Type:Journal Article
- Keywords:
Ferula sinkiangensis K. M. Shen;
DPPH free radical;
anti-oxidant activity;
ascorbic acid
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):80-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high-throughput detection of medicine on DPPH free radical scavenging activity;To analyze and preliminarily screen the anti-oxidant activity part of Ferula sinkiangensis. Methods Taking DPPH free radical scavenging rate of ascorbic acid as positive control, and IC50 as the evaluating criterion of DPPH free radical scavenging capability, oxidation resistance of different polar parts (petroleum ether part, ethyl acetate part, methyl alcohol part, and water part) of Ferula sinkiangensis was evaluated and screened. Results Different polarity parts of Ferula sinkiangensis DPPH radical scavenging of IC50 were:petroleum ether part 3252.22 μg/mL, ethyl acetate part 36.22 μg/mL, methyl alcohol part 32.22 μg/mL, water part 2643.38 μg/mL, and positive control group ascorbic acid 27.16 μg/mL. Conclusion The ethyl acetate and methyl alcohol parts of Ferula sinkiangensis were effective anti-oxidant activity site to eliminate DPPH free radicals. In this study, a simple, sensitive, and high-throughput detection method of DPPH radical scavenging assay was established to provide reference for the anti-oxidation medicine detection screening.
