Rapid Determination of Content of 3-Butylphthalide in Angelicae Sinensis Radix by Near-infrared Diffuse Reflection Spectrum
10.3969/j.issn.1005-5304.2015.03.024
- VernacularTitle:近红外漫反射光谱法快速测定当归中正丁基苯酞含量
- Author:
Yaya ZHANG
;
Zhirong GU
;
Yali WANG
;
Yujing SUN
- Publication Type:Journal Article
- Keywords:
near-infrared diffuse reflectance spectrum;
Angelicae Sinensis Radix;
3-butylphthalide
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quantitative model to rapidly determine content of 3-butylphthalide in Angelicae Sinensis Radix based on near-infrared diffuse reflectance spectroscopy (NIR). Methods Totally 145 batches of samples of Angelicae Sinensis Radix from 14 areas of Gansu and Yunnan Provinces were collected and dried in the shade. With the content of 3-butylphthalide determined by RP-HPLC-DAD as reference, the NIR spectra of 145 batches of Angelicae Sinensis Radix were collected by integrating sphere diffuse reflectance, and the PLS (partial least squares) quantitative analysis model was established between 7415-4056 cm-1. Results The Rc (correlation coefficient between actual content and predicted content), RMSEC (the root-mean-square error of calibration), RMSEP (the root-mean-square error of prediction), Rcv (cross-validation correlation coefficient), RMSECV (the root-mean-square error of cross-validation), and average predicted recovery reached 0.976 7, 0.614 3, 0.706 5, 0.897 8, 1.155 4 and 102.08%, respectively. Conclusion This prediction model was accurate, reliable, and can be used to quantitatively determine the content of 3-butylphthalide in Angelicae Sinensis Radix.
