Study on HPLC Characteristic Chromatogram of Shenshitong Granules
10.3969/j.issn.1005-5304.2015.03.026
- VernacularTitle:肾石通颗粒HPLC特征图谱研究
- Author:
Lili ZHOU
;
Xinhua XIA
;
Lin TANG
;
Yang DU
;
He DU
;
Wei YUE
;
Meng ZHANG
- Publication Type:Journal Article
- Keywords:
Shenshitong Granules;
HPLC;
characteristic chromatogram
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(3):98-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC characteristic chromatogram of Shenshitong Granules. Methods The chromatographie fingerprints were obtained through Thermo Hypersil GOLD-C18 column (4.6 mm×250 mm, 5μm) with the gradient elution solvent system composed of acetonitrile-0.2% phosphoric acid (0-20 min, 5%→10% acetonitrile;20-40 min, 10%→12%acetonitrile;40-60 min, 12%→14%acetonitrile;60-90 min, 14%→20%acetonitrile;90-120 min, 20%→28%acetonitrile). The detective wavelength was set at 280 nm;the flow rate was 1.0 mL/min;the column temperature was maintained at 30 ℃;the analysis time was 120 min. Results The HPLC characteristic chromatogram was built on basis of 10 batches of Shenshitong Granules, including 27 common peaks which contain the characteristic peaks of 6 Chinese herbal medicines, such as Radix Salvia Miltiorrhizae, Herba Lysimachiae, etc. Conclusion The established HPLC fingerprint has high sensitivity and good repeatability, and can be available for quality evaluation of Shenshitong Granules.
