Construction and characterization of invasion protein B gene deleted mutant of Salmonella typhimurium SL1344 strain
10.3969/j.issn.1000-484X.2015.02.016
- VernacularTitle:鼠伤寒沙门菌SL1344株侵袭性蛋白B缺失株的构建及生物学特性
- Author:
Songbiao CHEN
;
Jing LI
;
Chuan YU
;
Lei HE
;
Chunjie ZHANG
;
Xiangchao CHENG
;
Yinju LI
;
Yunfei YAN
;
Xiuzhe JIN
;
Guihua CHEN
;
Zhanqin ZHAO
- Publication Type:Journal Article
- Keywords:
Salmonella typhimurium SL1344;
sipB gene;
Deleted mutant;
Characteristics
- From:
Chinese Journal of Immunology
2015;(2):215-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to develop a safer vaccine strain exploit Salmonella typhimurium vaccine strain .A ΔsipB mutant of Salmonella typhimurium SL 1344 strain was constructed.Methods: Firstly, the recombinant suicide plasmid containing the missing 585 bp sipB ( PREΔsipB ) was built by homologous recombination , and screenned by two-step method.Results: PCR and sequencing results showed that SL 1344ΔsipB was successfully constructed.It was no significant changes compared with SL 1344.But compared with the parent strains SL 1344 , the mutant strain had obvious change in its virulence , oral challenge of bacteria in mice revealed that LD50 of the mutant strain was 1.70 ×108 CFU,the toxicity reduced about 1.4%.The protection rate induced by the sipB mutant was 50%,and the serum antibody peaked 14 d post-immunization.Conclusion:The SL1344ΔsipB mutant was constructed suc-cessfully,and genetic stability ,significantly reduced virulence.The study provides a new approach for further study of the relationship between the gene and pathogenicity of Salmonella typhimurium.It is likely that the ΔsipB mutant could be adapted to develope attenuated Salmonella vaccine.
