Clinical value analysis of serum protein fingerprint in patients with rheumatoid arthritis associated interstitial lung disease
10.3969/j.issn.1000-484X.2015.02.020
- VernacularTitle:类风湿关节炎并发间质性肺疾病患者血清蛋白质指纹图谱的临床价值分析
- Author:
Yanmei WU
;
Shouchun PENG
;
Jian SHEN
;
Jing WU
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis associated interstitial lung disease;
Rheumatoid arthritis;
Health adult;
Serum;
SELDI-TOF-MS
- From:
Chinese Journal of Immunology
2015;(2):236-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen differentially expressed proteins in serum proteomic patterns in patients with Rheumatoid Arthritis associated Interstitial Lung Disease by Surface enhanced laser desorption /ionization mass spectrometry with Protein Chip ,and evaluate the value of a preliminary study.Methods: WCX2 ( Weak cation exchange chip ) and Surface enhanced laser desorption/ionization mass spectrometry with ProteinChip were performed to test serum proteins in 19 Rheumatoid Arthritis associated Interstitial Lung Disease,15 Rheumatoid Arthritis patients and 13 health adults,Biomarker Wizard ,Biomarker Pattern and Spss13.0 software were used in combination to analyze the data.Results:The mass/charge ratio ( M/Z) value of 142 protein peaks were detected in serum , there were 4 proteins differentially expressed with statistical significance among them ( P<0.05;Student′s t-test ) , which were the proteins with M/Z 3382.59 ,3453.39 ,11886.0 ,5825.48;the proteins with M/Z 11689.4 ,2266.49 ,1020.22 ,4392.28 ,5074.38 and 2764.69 were selected to establish the optimal diagnostic model.The sensitivity was of was 90%(9/10),the specificity was of 90%(18/20).Conclusion: Surface enhanced laser desorption/ionization time of flight mass spectrum can screen out the significantly different proteins in serum of patients with Rheumatoid Arthritis associated Interstitial Lung Disease.As bomarkers , both of their sensitivity and specificity were high and determination have good prospects for clinical application .
