Regulation of mesenchymal stem cells expressing Notch signaling pathway of asthma
10.3969/j.issn.1000-484X.2015.02.003
- VernacularTitle:间充质干细胞对哮喘大鼠肺组织Notch信号通路的表达调控
- Author:
Lijun TIAN
;
Tingting HAN
;
Meng GAO
;
Fuling WU
;
Xuebin FENG
;
Yuesi WANG
- Publication Type:Journal Article
- Keywords:
Asthma;
Mesenchymal stem cells;
Notch signaling pathway;
Transplantation
- From:
Chinese Journal of Immunology
2015;(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the regulation of MSCs on the asthma;and to observe the expression of Notch signal in lung tissue of asthmatic rats.Methods:30 rats were randomly divided into 3 group :normal control group , asthma model group and MSC transplantation group.Making paraffin sections of lung tissue for pathological examination ,quantitative real-time PCR(RT-PCR) were used to identify the expression of Notch2 and Jagged1 mRNA in the rat lung tissue,and the expression of Notch2,Jagged1 were measured by Western blot.Results:Compared with normal control group and MSC transplantation group ,inflammatory cell infiltration and narrower airway were observed in asthma model group .The lever of Notch2,Jagged1 in asthma model group was higher than MSC transplantation group and normal control group ( P<0.05 ).Conclusion:Mesenchymal stem cells can affect the expression of the Notch signaling pathway in asthmatic rats ,and play a role in the treatment of asthma.
