Effects of ceftriaxone on glutamate and glutamate transporter subtype-1 in hip-pocampus following traumatic brain injury in rats

VernacularTitle:头孢曲松对脑损伤后海马谷氨酸及谷氨酸转运体-1的影响
Author: Li RAO ; Jing YANG ; Zhequn SHI
Publication Type:Journal Article
Keywords: Ceftriaxone; TBI; Brain edema; Glu; GLT-1
From: Chinese Journal of Immunology 2015;(2):176-179
CountryChina
Language:Chinese
Abstract: Objective:To sduty the effect of β-lactam antibiotics efiriaxone on the levels of glutamate and glutamate transporter subtype-1 in hippocampus following traumatic brain injury in rats.Methods: All rats were divided randomly into three groups:sham group;TBI group and CTX group.The rat model of TBI were made by modified Feeney method ,and treated with ceftriaxone immediately after injury ( 200 mg/kg ).Wet-dry weight method was used to evaluate brain edema.The content of glutamate in hippocampus was measured with the high performance liquid chromatography.The expression of GLT-1 in hippocampus areas was tested by immunohisto-chemical and Western blot methods.Results:Compared with TBI group ,TBI-induced cerebral edema was significantly attenuated ( P<0.05 ) , the content of glutamate in hippocampus was were significantly decreased ( P<0.05 ) , the level of GLT-1 were significantly increased in CTX group ( P<0.05 ).Conclusion: β-lactam antibiotics ceftriaxone can block the excitatory neurotoxicity , reduce the extent of brain edema.