Effects of Ophiocordyceps xuefengensis on proliferation of DC-CIK cells and activity of killing HepG-2 cells by DC-CIK cells
10.3969/j.issn.1000-484X.2015.02.011
- VernacularTitle:雪峰虫草对DC-CIK增殖及HepG-2细胞杀伤作用的实验研究
- Author:
Bing ZHENG
;
Fangyi XIE
;
Guohui CAI
;
Rucai ZHU
;
Ke LI
;
Shouquan GAO
;
Dianbo TAN
;
Xiaoyong HAO
;
Yuhui QIN
- Publication Type:Journal Article
- Keywords:
Ophiocordyceps xuefengensis;
Dendritic cells;
Killer cells;
HepG-2 cells
- From:
Chinese Journal of Immunology
2015;(2):189-192
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Ophiocordyceps xuefengensis on proliferation of DC -CIK cells and the activity of killing HepG-2 cells by DC-CIK cells.Methods:Peripheral blood mononuclear cells were routinely isolated and induced into DC and CIK.DC and CIK co-cultured by 1∶5 for 7 days,then Ophiocordyceps xuefengensis were added into medicine group ,observed the mor-phology of the cells on the tenth day and counted the DC-CIK number of each group.DC-CIK cells acted as effector cells and the HepG-2 cells as target cells , cck-8 method for the detection of DC-CIK in the killing rate of HepG-2.Results: The Ophiocordyceps xuefengensis was able to proliferate the DC-CIK dramatically ,the optimal concentration was 0.1 mg/ml.Cultivation of Ophiocordyceps xuefengensis induced DC-CIK cells on HepG-2 cells killing effect was better than that of the routine method of DC-CIK cells; the effection of Ophiocordyceps xuefengensis killing HepG-2 cells was not obviously.Conclusion: Ophiocordyceps xuefengensis can enhance the anti-tumor activity of DC-CIK mainly by promoting the proliferation of it.
