Effect of immunity-related GTPase Irgm1 gene knockout on CD4+T cell subsets in experimental autoimmune encephalomyelitis
10.3969/j.issn.1000-484X.2015.02.009
- VernacularTitle:Irgm1基因敲除对实验性自身免疫性脑脊髓炎小鼠CD4＋T细胞亚群的影响
- Author:
Caihong WANG
- Publication Type:Journal Article
- Keywords:
Multiple Sclerosis ( MS );
Experimental autoimmune encephalomyelitis ( EAE );
Immunity related GTPase1 ( Irgm1);
CD4+T cell Subsets
- From:
Chinese Journal of Immunology
2015;(2):180-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate whether Irgm 1 impact CD4+T cell subsets in the experimental autoimmune encephalomyelitis.Methods: The Irgm1 heterozygous mice were backcrossed with C 57BL/6 Wt.mice for 10 generations to produce C57BL/6 Irgm1+/-mouse.C57BL/6 Irgm1+/-mice were intercrossed to obtain three genotypes:Irgm1-/-, Irgm1+/-and Irgm1+/+.To establish model of EAE ,C57BL/6 Wt.mice and Irgm1 knock out mice were immunized with myelin oligodendrocyte glycolprotein 33-55 ( MOG33-55 ) and the clinical symptoms were observed.The proliferation of lymphocytes to MOG antigen was detected with Methyl Thiazolyl Tetrazolium ( MTT).The infiltration of inflammatory cells in the spinal cords was observed through HE staining.The CD4+T cell subsets from lymph nodes ,spleens and CNS were detected by flow cytometry.Results:EAE model was induced successfully.The proliferation of T cells in lymph nodes in Irgm 1-/-mice was lower than Wt.mice.Quantitative analysis of flow cytometry indicates that , compared with Wt.mice,the level of Th1 subset was higher,Th17 was lower relatively in lymph nodes and CNS of Irgm1 knock out mice.Conclusion:Irgm1 knockout mice can be partially protected EAE spinal cord function and clinical symptoms .Irgm1 may play a key role at early stage of EAE ,which may use as an important molecular target for treatment of EAE.
