Expression of early growth response factor 1 and tissue factor in rats with carotid artery injury
10.3969/j.issn.2095-4344.2015.27.006
- VernacularTitle:构建颈动脉损伤模型大鼠早期生长反应因子1及组织因子的表达
- Author:
Xi ZHANG
;
Chi MIAO
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Transfection;
Carotid Arteries;
Genes
- From:
Chinese Journal of Tissue Engineering Research
2015;(27):4293-4298
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: DNA enzyme targeting early growth response factor 1 (Egr-1) mRNA (ED5) can inhibit expression of downstream target genes by specificaly inhibiting expression of early growth response factor 1. OBJECTIVE:To investigate the effects of ED5 on the expression of plasma tissue factor after vascular baloon injury in rats and the mechanism of inhibiting neointimal hyperplasia. METHODS: Intimal injury models of the left carotid artery were made in rats. Then, ED5, MgCl2 and FuGene6 were injected into the injured vascular segment. RESULTS AND CONCLUSION:At days 3, 7, 14, 21, the expression levels of Egr-1 and tissue factor in plasma were significantly down-regulated in the ED5 transfection group compared with the MgCl2 and FuGene6 groups (P < 0.01); and neointimal hyperplasia was significantly inhibited by ED5 at days 7, 14 and 21 after modeling (P < 0.01). ED5 may inhibit neointimal hyperplasia folowing baloon injury of rat common carotid artery through down-regulation of tissue factors.
