The protective effect of TanshinoneⅡA on rat models of uterine ischemia/reperfusion injury
10.3969/j.issn.2095-4344.2015.27.022
- VernacularTitle:丹参酮ⅡA对子宫缺血再灌注损伤模型大鼠的保护效应
- Author:
Dong WANG
;
Xiaowei JI
;
Shuang ZHANG
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Animal Model;
Uterus
- From:
Chinese Journal of Tissue Engineering Research
2015;(27):4384-4388
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The phenomenon of temporary ischemia of the tissues and organs caused by injury is common in the clinic. However, there are few studies on the treatment of ischemia/reperfusion injury. It is significant to investigate the effect of TanshinoneⅡA on improving uterine ischemia/reperfusion injury in a rat model. OBJECTIVE:To observe the effect of TanshinoneⅡA intervention on improving uterine ischemia/reperfusion injury in rats. METHODS:The rat models of uterine ischemia/reperfusion injury were established by suture method. The rats that were intraperitonealy injected with Sodium Tanshinone IIA Silate injection (20 mg/kg) at 5, 3, 1 days and 30 minutes before the operation were included in the experimental group. Model and sham operation groups were established for comparison. RESULTS AND CONCLUSION:Spectrophotometry and immunohistochemical staining results showed that at 1 and 3 hours of ischemia/reperfusion, the malondialdehyde concentration and Bax protein expression in the injuried rat uterine tissue were significantly decreased (P < 0.05), superoxide dismutase (SOD) activity and Bcl-2 protein expression were significantly increased (P < 0.05) in the experimental group than in the model group. The results confirm that TanshinoneⅡA has a protective effect on uterine ischemia/reperfusion injury in rats.
