Intervention of inflammatory cell infiltration and cartilage destruction of the knee joints in mouse models of collagen-induced arthritis by small molecule tyrosine kinase inhibitors
10.3969/j.issn.2095-4344.2015.24.003
- VernacularTitle:酪氨酸激酶抑制化合物干预胶原诱导关节炎模型炎性细胞浸润及软骨破坏
- Author:
Wei LIU
;
Yong ZHANG
;
Dechun GENG
;
Lixin HUANG
;
Jian LI
- Publication Type:Journal Article
- Keywords:
Arthritis;
Osteoarthritis,Knee;
Protein-Tyrosine Kinases;
Cartilage
- From:
Chinese Journal of Tissue Engineering Research
2015;(24):3783-3787
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:At present, spleen tyrosine kinase is the new target of studying and treating rheumatoid arthritis. OBJECTIVE:To study the influence of smal molecule tyrosine kinase inhibitor HL131078 on the inflammatory cel infiltration and cartilage destruction of the knee joint of mice with col agen-induced arthritis. METHODS:Forty DBA/1 mice were randomly and evenly divided into blank, model, positive and experimental groups. Col agen type II (CII) solution and Freund’s complete adjuvant (including mycobacterium tuberculosis) were injected into the mice of the latter three groups through the tail to establish mouse models of col agen-induced arthritis. At 2 weeks after the the first immunization with CII, the mice in the positive group were intragastrical y given R406 (10 mg/kg), once a day, for 28 consecutive days. The mice in the experimental group were intragastrical y given HL131078 (10 mg/kg), once per day, for 28 consecutive days. RESULTS AND CONCLUSION:Compared with the model group, the mean arthritis indexes of mice in the experimental and positive groups started to decline at 29 and 26 days. In the experimental group, the cartilage destruction of mouse knee joint was obviously reduced and the inflammatory cel infiltration in the knee joints was obviously reduced, which was close to that in the positive group. The results demonstrate that the smal molecule tyrosine kinase inhibitor HL131078 can effectively reduce inflammatory cel infiltration and cartilage destruction in the knee joints of mice with col agen-induced arthritis.
