Expression of interleukin-17 in the periodontal tissue of alveolar resorption-induced periodontitis rats
10.3969/j.issn.2095-4344.2015.24.007
- VernacularTitle:牙槽骨吸收牙周炎模型大鼠牙周组织和血清白细胞介素17的表达
- Author:
Jiaxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Periodontitis;
Lipopolysaccharides;
Interleukin-17;
Rats
- From:
Chinese Journal of Tissue Engineering Research
2015;(24):3803-3807
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have found that interleukin-17 secreted from T helper cel s 17 has a high expression in the serum and injured tissues of periodontitis patients, but the specific relationship is stil unclear. Therefore, an appropriate animal model is necessary to analyze the expression of interleukin-17 in model rats. OBJECTIVE:To analyze the expression of interleukin-17 secreted from T helper cel s 17 in experimental periodontitis rat. METHODS:Rats were divided into three groups:the chronic periodontitis model in ligation group was established by ligaturing the first molar on the left maxil ary, and rats were kil ed 8 weeks later;the alveolar resorption model in lipopolysaccharide group was established by injecting lipopolysaccharide, and rats were anesthetized and kil ed 20 days after the model was completed;rats in control group did not receive any disposal. RESULTS AND CONCLUSION:Under light microscope, the number of osteoclasts in the ligation group and lipopolysaccharide group was significantly higher than that in the control group (P<0.05). The rats in the ligation group had swol en and red gums, as wel as periodontal pocket. Immunohistochemical results showed that the expression levels of interleukin-17 in the ligation group and lipopolysaccharide group were both higher than that in the control group (P<0.05). ELISA results showed that there was no difference in the serum concentration of interleukin-17 among three groups (P>0.05). These findings indicate that interleukin-17 exerts an important role in the alveolar resorption, and T helper cel s 17 may be involved in the local periodontal inflammation but have weak effects on the system inflammation.
