Activated expression of matrix metalloproteinase is involved in the proliferation of MC3T3-E1 cells induced by lipopolysaccharide
10.3969/j.issn.2095-4344.2015.24.018
- VernacularTitle:脂多糖激活基质金属蛋白酶表达诱导MC3T3-E1细胞的增殖
- Author:
Guanghua YAN
;
Hua QIN
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Matrix Metal oproteinases;
Osteoblasts
- From:
Chinese Journal of Tissue Engineering Research
2015;(24):3865-3869
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Matrix metal oproteinases and their inhibitors are proteolytic enzymes contaning Zn+, and involved in extracel ular matrix degradation and tissue remodeling of a variety of tissues. OBJECTIVE:To observe the effects of matrix metal oproteinases in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide. METHODS:MC3T3-E1 cel line was divided into four groups randomly:control group, low-dose lipopolysaccharide group (1μmol/L), moderate-dose lipopolysaccharide group (10μmol/L), and high-dose lipopolysaccharide group (100μmol/L). The proliferation rate in each group was analyzed. Matrix metal oproteinase 2, 3, 9 and matrix metal oproteinase inhibitor 1 and 2 expressions were detected. RESULTS AND CONCLUSION:The proliferation rate was increased greatly after medication of lipopolysaccharide in time-dependent and concentration-dependent manners. Moreover, the expressions of matrix metal oproteinases and their inhibitors were apparently enhanced, and showed significant differences. Results indicate that the enhanced expressions of matrix metal oproteinases participated in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide.
