Dendritic cell costimulatory molecule expression and immunosuppressant intervention
10.3969/j.issn.2095-4344.2015.24.020
- VernacularTitle:树突状细胞共刺激分子表达与免疫抑制剂的干预
- Author:
Xiaoyong YANG
;
Qingchun YAO
;
Wei WANG
;
Man QI
;
Xiying HONG
;
Chenmei LIU
;
Hang YIN
;
Hang LIU
;
Liang REN
;
Xiaopeng HU
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Dendrites;
Rapamycin;
Lymphocytes
- From:
Chinese Journal of Tissue Engineering Research
2015;(24):3876-3881
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies on immunosuppression and anti-rejection after organ transplantation mainly focused on effects of T lymphocytes-mediated immune response and immunosuppressive agents on T lymphocytes. Effects of dendritic cel s were unclear. The manifestation and mechanism of immunosuppressive agent effects on dendritic cel s are not identical. OBJECTIVE:To compare the effects of different immunosuppressive agents on expression and function of costimulatory molecules of dendritic cel s, and to explore the mechanism of action of immunosuppressive agents. METHODS:20μg/L rapamycin, 0.04 mg/L mycophenolate, 10μg/L tacrolimus and 1 mg/L cyclosporine A were separately added during bone marrow cel s of C57BL/6 mice were differentiated into dendritic cel s. RESULTS AND CONCLUSION:Flow cytometry results revealed that CD40 expression in each group:rapamycin0.05). One-way mixed lymphocyte reaction results displayed dendritic cel costimulatory T cel proliferation in each group:rapamycin
