Expression of cytokines in patients with chronic myelocytic leukemia

VernacularTitle:慢性粒细胞性白血病患者多种细胞因子表达的对比研究
Author: Yingya WU ; Jishi WANG ; Qing FANG
Publication Type:Journal Article
Keywords: Leukemia,myeloid,chronic; Protein army analysis; Microchip analytical procedures; Cytokines
From: Journal of Leukemia & Lymphoma 2010;19(3):153-155
CountryChina
Language:Chinese
Abstract: Objective To investigate expression of 7 kinds of immune related human cytokines (IL-2, IL-4, IL-12, IL-13, IFN-γ TNF-α, MIP-1α) in CML patients. Methods Pure monoclonal antibody on the prepared NC membrane glass slides under certain environmental condition to make human eytokines protein microarray were spotted and serum samples (25 patients, 25 the normais) were collected. Cytokines concentration in the serum with the protein microarray were tested by ELISA. Results IL-4 and IL-12 serum concentration in CML patients are lower than that of the normal (P <0.05). However. No statistic difference of MIP-1α was found between CML patients the normal. Conclusion Cell mediated immunity and humoral immunity of CML patients are both inhibited in some extent and the expression of MIP-1α may be inhibited by p210 in CML patients.