Dimethyl sulfoxide acts as a protective agent to perfuse rabbit amputated limbs:the relative recovery of local drug concentrations
10.3969/j.issn.2095-4344.2015.24.016
- VernacularTitle:二甲基亚砜充当保护剂灌注兔离段肢体局部药物浓度的相对回收率
- Author:
Zhuoling AN
;
Kun DING
;
Chunsheng LIU
;
Zexing ZHU
;
Jingchao ZHOU
;
Zhen ZHANG
;
Shuming ZHANG
;
Dan WANG
- Publication Type:Journal Article
- Keywords:
Dimethyl Sulfoxide;
Microdialysis
- From:
Chinese Journal of Tissue Engineering Research
2015;(24):3855-3859
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The effect of dimethyl sulfoxide cryoprotectants has been got a lot of verification in the low-temperature medical applications. But there is no literature addressing microdialysis detection of dimethyl sulfoxide cryoprotectants. OBJECTIVE:To investigate the microdialysis relative recovery of different concentrations of dimethyl sulfoxide cryoprotectants used for limb reattachment. METHODS:In vitro linear probe relative recovery of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) was detected by retrodialysis gain and loss method. The correction in vivo experiment was done to estimate dimethyl sulfoxide relative recovery in rabbit amputated limbs. RESULTS AND CONCLUSION:The relative recoveries of different concentrations of dimethyl sulfoxide (2%, 5%, 8%) were (49.49±3.56)%, (46.30±1.48)%, (52.66±2.54)%using retrodialysis gain method and (50.99±6.89)%, (43.86±1.35)%, (50.67±0.75)%using retrodialysis loss method. The average recoveries were (49.48±3.18)%and (48.51±4.03)%, respectively. There was no difference in the relative recovery of dimethyl sulfoxide detected using two methods. The change of dimethyl sulfoxide concentration could not influence the retrodialysis gain and loss method calibration results. The recovery was (15.45±4.8)%in vivo. These findings indicate that the microdialysis technology is suitable for dimethyl sulfoxide sampling in vivo that has no obvious influence on the relative recovery.
