Involvement of MRE11 in inflammasome activation:a preliminary research
10.7644/j.issn.1674-9960.2015.01.008
- VernacularTitle:MRE11参与炎症小体信号通路激活的初步研究
- Author:
Jun JIANG
;
Qinfang HAO
;
Deyong ZOU
;
Liping ZHANG
;
Xiaoli ZHANG
;
Xiaoxing GE
;
Xiaoli YANG
- Publication Type:Journal Article
- Keywords:
MRE11;
RNA,small interfering;
interleukin-1 beta;
inflammasome activation
- From:
Military Medical Sciences
2015;(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the function of MRE11 in inflammasome activation.Methods Different stimuli,in-cluding Poly(I∶C), Poly(dA∶dT),E.coli gDNA,293T gDNA,CPPD and HSV,were used to identify the effective inflamma-some activator using ELISA.Then, MRE11 siRNA oligos were sythesized and transfected into THP-1 cells while Western blotting was used to analyze the efficacy of MRE 11 knockdown .Finally ELISA and Western blotting were used to analyze the involvement of MRE11 in inflammasome activation induced by Poly (I∶C), Poly(dA∶dT), E.coli gDNA and 293T gDNA. Results The IL-1βsecretion and pro-caspase-1 activation which induced by Poly ( I∶C) , Poly( dA∶dT) , E.coli gDNA and 293T gDNA were reduced with different degrees in MRE 11-knockdown THP-1 cells.Conclusion These results indicate that MRE11 is required for inflammasome activation induced by genetic materials .
