Protective effects of penehyclidine hydrochloride on A549 cells against oxidative injury
10.11958/j.issn.0253-9896.2015.07.005
- VernacularTitle:盐酸戊乙奎醚对肺泡Ⅱ型上皮细胞氧化损伤的影响
- Author:
Xueke DU
;
Linghui PAN
;
Shenglin PEI
;
Wanyun GE
- Publication Type:Journal Article
- Keywords:
penehyclidine hydrochloride;
lung epithelial typeⅡcells;
A549;
oxidative stress damage
- From:
Tianjin Medical Journal
2015;(7):721-723
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of penehyclidine hydrochloride (PHC) on lung epithelial type Ⅱcells against oxidative stress damage. Methods A549 cells treated with H2O2 were used as oxidative stress damage cell model. A549 cells were divided into 3 groups: control group (C group), H2O2 treated group (H group) and PHC treated group (P group). The viability of A549 cells was measured by MTT assay. The apoptotic rate was measured by TUNEL assay. The lev?els of malonicdialed (MDA), reactive oxygen species (SOD), reduced glutathione hormone (GSH) and nicotinamide adenine dinucleotide phosphate (NADPH) of cells were detected by biochemistry colorimetry. Results Compared with group C, the viability of A549 and the contents of SOD, GSH and NADPH were significantly decreased in group H, while MDA content and apoptotic rate were increased (P<0.05). Compared with group H, the viability of A549, the contents of SOD, GSH and NADPH were significantly increased in group P, while MDA content and apoptotic rate were reduced (P<0.05). Conclu?sion Penehyclidine hydrochloride shows protective effects on A549 cells through reducing the oxidative damage induced by H2O2.
