Baicalin induces osteogenic differentiation of rat bone marrow derived mesenchymal stem cells via Wnt/β-catenin signaling pathway
10.3969/j.issn.1001-1978.2015.07.007
- VernacularTitle:黄芩苷通过Wnt/β-catenin信号通路对大鼠骨髓间充质干细胞成骨分化的促进作用
- Author:
Chenrui LI
;
Zhiyuan MENG
;
Yinbo NIU
;
Yuankun ZHAI
;
Yalei PAN
;
Li XIE
;
Qibing MEI
- Publication Type:Journal Article
- Keywords:
baicalin;
rat bone marrow derived mesen-chymal stem cells(rBMSC);
osteogenic differentia-tion;
Wnt/β-catenin signaling pathway;
alkaline phos-phatase
- From:
Chinese Pharmacological Bulletin
2015;(7):919-924
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of Wnt/β-cate-nin signaling pathway on the baicalin-induced osteo-genic differentiation in rat bone marrow derived mesen-chymal stem cells ( rBMSC ) . Methods rBMSC was isolated and cultured by adherence screening method. Alkaline phosphatase ( ALP) amount, CFU-FALP and mineralized nodules were compared between each ba-icalin group and vehicle control group at different time points. Real time q-PCR was employed to evaluate the mRNA level of Wnt signaling-related marker ( Wnt10a, GSK-3β,β-catenin and LEF1) after baica-lin treatment. Protein expression of β-catenin and Runx2 was measured by Western blot. Results Ba-icalin significantly increased ALP activities from day 3 to day 7 . The formation of CFU-FALP and mineralized nodules remarkably increased after rBMSC was treated with1, 10, 50 μmol · L-1 baicalin. mRNA levels of Wnt10a, β-catenin, GSK-3β, LEF1and osteocalcin were enhanced significantly in baicalin-treated group compared to control group. Protein expression of β-catenin and Runx2 was also elevated. Conclusion Baicalin ( 0. 1 to 50 μmol · L-1 ) promotes the osteo-genic differentiation and maturation of rBMSC, in which Wnt/β-catenin signaling pathway might be in-volved.
