Expression of hippocampus NGF in MPTP mouse model of Parkinson′s disease

VernacularTitle:MPTP致帕金森模型小鼠海马区NGF基因的表达研究
Author: Li CAO ; Yanqiu XIA ; Xin LIU ; Ling XU ; Xiangxian WANG ; Jun LU ; Yuanlin ZHENG
Publication Type:Journal Article
Keywords: MPTP; Parkinson′s disease; TH; hippo-campus; NGF; Cognitive ability
From: Chinese Pharmacological Bulletin 2015;(7):957-961
CountryChina
Language:Chinese
Abstract: Aim To detect the expression of hippocam-pus NGF gene in the mouse model of Parkinson 's dis-ease. Methods By intraperitoneal injection of 1-meth-yl-4-phenyl-1,2, 3, 6-tetrahydropyridine ( MPTP), a mice model of Parkinson ’ s disease was established. The success of PD model was identified by detecting the expression of mesencephalic tyrosine hydroxylase ( TH) with Western blot and immunofluorescence. The movement and cognitive ability of mice were evaluated by foot print analysis and step-through passive avoid-ance test. The expression of NGF gene in hippocampus of mice was detected with RT-PCR and in situ hybrid-ization. Results Compared with the control group, the levels of TH and NGF were reduced in the experi-mental group and the behavioral indexes were deflected significantly. Conclusion NGF may play an impor-tant role in the occurrence and development of the PD cognitive disorder.